Abstract
Genetic engineering is a complex process that refines genes at the molecular level. The field of industrial biotechnology relies much on genetic engineering techniques which have played a significant role in the improvement of this area during the past few years. The discovery and characterization of clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has brought a breakthrough in genome-editing techniques owing to its high versatility and efficiency. CRISPR/Cas systems are bacterial and archaeal adaptive immune defense systems repurposed as versatile genetic editing tools in a broad range of microorganisms. The technique has potential for numerous microbial engineering applications, including microbial strain typing, autoimmunity, culture immunization, and engineering metabolic pathways for improved synthesis of biochemicals. At present, the RNA-guided endonuclease CRISPR/Cas9 (CRISPR-associated protein 9) approach is also used to engineer various microbes for industrial applications. This chapter focuses on the recent trends of CRISPR/Cas9 approach in editing the genome of industrially important microorganisms for producing various value-added chemicals and compounds.
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Sarkar, S., Yadav, M., Kumar, A. (2023). CRISPR/Cas9 System: An Advanced Approach for the Improvement of Industrially Important Microorganisms. In: Verma, P. (eds) Industrial Microbiology and Biotechnology. Springer, Singapore. https://doi.org/10.1007/978-981-99-2816-3_4
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